罗丹明B的飞秒双光子荧光显微成像研究

Research on Femtosecond Two-photon Fluorescence Microscopy of Rhodamine

摘要:

利用宽带的飞秒振荡器作为激发光源搭建了双光子荧光显微成像系统,在飞秒脉冲的宽带激发下,测量了罗丹明B溶液的双光子荧光光谱,开展了罗丹明B固体样品的双光子荧光显微成像研究,在双光子荧光显微成像研究中,通过调节激发脉冲的功率,分析了双光子荧光强度和激发脉冲功率之间的关系,并且利用半波片改变线偏振激发光场的偏振方向,研究了双光子荧光强度随激发脉冲偏振方向的变化趋势,实现了双光子荧光强度的类正弦调制。

Abstract:

With broadband femtosecond (fs) laser as the excitation source, two-photon fluorescence (TPF) imaging system is built. Under broadband excitation of fs pulses, TPF spectrum of Rhodamine B solution is measured and TPF microscopy is performed with solid Rhodamine B samples. In the TPF microscopy of solid Rhodamine B sample, relationship between TPF intensity and power of the excitation pulses is analyzed. Besides, TPF intensity is modulated through adjusting polarizationSdirection of the linearly polarized exciting field with a half wave plate and sine-like modulation of TPF intensity is realized.