Abstract:A microscopy image automatic acquisition system for space life science is introduced in this thesis,it can get the cell grade microscopy image of the three dimensional target.The dissimilarity between camera automatic focusing and microscopy automatic focusing is analysed,the microscopy imaging acquisition method of 3-D target applied in complex condition is put forward and the implementation method is given.The experimental results show that the system can accurately acquire the microscopy image of the three dimensional target, and the image is distinct and unanimous.