The design of a scientific grade CCD camera with high sensitivity and refrigeration was introduced. The time driving, camera control and transmission data of the camera were completed by the FPGA chip. The correlated double sample circuit was designed to reduce the reset noise. The internet port chip W5300 sent the data to image capture software by TCP/IP protocol. The camera was refrigerated by semiconductor refrigerator in combination with water cooling method. The result of the experiment verified that the lowest luminance of the camera could be 0.005Lx when its temperature was -20℃. So the camera has the feature of high sensitivity to satisfy the requirement in low light level environment.